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Q1. What foods can be tested for fat content by Soxhlet extractor?
Answer: GB5009.6-2016 food safety National Standard Determination of Fat in Food has four detection methods, namely1. Soxhlet extraction method, applicable to the determination of free fat content in food; 2. Acid hydrolysis method, applicable to the determination of total fat content in food; 3. Alkali hydrolysis method, applicable to the determination of fat in milk, dairy products and infant formula food; 4. Gabor method, applicable to the determination of fat in milk, dairy products and infant formula food.Therefore, the fat content of milk, dairy products and infant formula foods, such as yogurt, milk powder and cheese, should be detected by alkaline hydrolysis or Gabor method, mainly by liposuction bottle or Gabor creamer; other samples except milk, dairy products and infant formula foods should be detected by Soxhlet extraction or acid hydrolysis method, both of which require Soxhlet extractor.
Q2. How to select the appropriate seal according to the organic solvent in the experiment?
Answer: Due to the different standards and experimental methods for different samples, it is necessary to select the corresponding organic solvent for the experiment. Organic solvents commonly used in Soxhlet extraction experiments include petroleum ether, ether, acetone, n-hexane and other single or mixed reagents. Generally, the Soxhlet extractor uses fluororubber and butyl rubber as sealing materials, of which fluororubber is resistant to non-polar reagents and butyl rubber is resistant to polar reagents. However, if mixed reagents are used, sealing materials such as polytetrafluoroethylene, which can withstand all solvents, should be selected. (Note: need to communicate with the factory engineer)
Q3. Can results be obtained directly by putting the samples on the machine?
Answer: The current Soxhlet extractor is an experimental test tool for sample extraction, which mainly completes the sample extraction process. Before and after extraction, it is necessary to weigh or quantify and calculate the target substance with the help of other test instruments (such as titrator, high performance liquid chromatograph, spectrophotometer, etc.).
Q4. Can organic solvents be recycled?
Answer: Yes. Now the automatic instrument has the function of automatic solvent recovery. After the experiment, the solvent is automatically recovered into the solvent bottle, which can be reused after treatment.
Q5. How to prevent the leakage of organic solvents such as ether from harming laboratory personnel and the environment?
Answer: Regular instruments should have built-in high-sensitivity organic solvent leakage detection devices such as ether. When the leakage of organic solvents such as ether reaches the threshold of normal occupational regulations, the instrument will automatically alarm to ensure the safety of laboratory personnel.